Yeast surface display (YSD) technology has become a powerful tool for protein engineering to display the desired target protein on the surface of yeast via genetic fusion to an anchor protein. Lots of yeast cell wall proteins are identified, including Agα1p, Aga2p, Cwp1p, Cwp2p, Tip1p, Flo1p, Sed1p, YCR89w, and Tir113. Selecting an anchor protein is a crucial consideration for the total amount of protein displayed and expressed, and it can enhance the display efficiency or functional properties.
• They must have a strong anchoring domain to ensure that exogenous proteins can be immobilized on the surface.
• They must be compatible with foreign proteins, be able to form fusion proteins, and should not be able to interact with each other.
• They must be resistant to protease hydrolysis.
In addition, an ideal anchor protein contains a signal sequence that mediates its efficient transport to the cell surface, where it strongly immobilizes the fused target protein without interference with the stability or activity of the displayed protein. The epitope tags enable quantification of fusion protein expression levels on each cell by flow cytometry using fluorescently labeled antibodies.
Fig.1 Schematic illustration of yeast surface display.
Yeast surface display has two extraordinary advantages relative to phage and bacteria display technologies. First, yeast is a eukaryotic expression system capable of incorporating post-translational modifications. Second, the heterologous protein can be fused at anchor protein without disrupting its core structure, thus without undermining its surface displaying efficiency.
Yeast display technology has been utilized for a variety of biotechnology and biomedical applications, such as electrochemical devices, biocatalysis, bioadsorption and bioremediation, increase of resistance to organic solvents, protein-protein and protein-ligand interaction, vaccine generation, antibody engineering, biosensor design, bioethanol production.
Fig.2 Applications of yeast surface display.
• We have many years of service experience in cell surface display.
• We have a world-class service platform.
• The price of Microbiosci is very competitive in the industry and the price is favorable.
• One-stop service.
• Ensure efficient service, and urgent service can be provided.
• Periodic progress updates.
• SDS-PAGE Analysis
• Western Blot Analysis
• Enzyme assays
• Fluorescence assays
• Flow cytometry
• Immunofluorescence microscopy
In general, our turnaround time is 4-8 weeks depending on the size of your project.
Microbiosci is a company specialized in microbial and molecular services. Microbiosci offers stable, efficient and successful yeast display technology for recombinant protein production at industrial scale. With the rich experience, our talented scientists advise appropriate construction strategy in various biotechnological applications. Know more about how our experienced team can facilitate your work, please feel free to contact us at firstname.lastname@example.org or 1-631-626-9181.
1. Andreu, C., & del Olmo, M. lí. (2018). Yeast arming systems: pros and cons of different protein anchors and other elements required for display. Applied Microbiology and Biotechnology, 102(6), 2543-2561.
2. Cherf, G. M., & Cochran, J. R. (2015). Applications of Yeast Surface Display for Protein Engineering. Methods in molecular biology (Clifton, N.J.), 1319, 155-175.