Water Microbiome Analysis

To better understand microbial dynamics, we characterized and compared the bacterial community of water using rRNA surveys, metagenomics, metabolomics, proteomics, which are good tools to elucidate composition of microbial communities residing in various aquatic environments.

Water Microbiota Analysis

Sewage Microbiome Analysis

Sewage contains diverse microbes, and regular microbiological screening has become key factor to monitoring the wastewater treatment and protecting environmental health.

Freshwater lake Microbiome Analysis

Freshwater lakes in diverse regions provide indispensable water resources for humans. The microbial communities in freshwater lake ecosystem play a central role in global environmental and public health perspectives.

Rainwater Microbiome Analysis

Excessive rainfall is a critical origin to historical waterborne disease outbreaks owing to mobilization and transport of bacterial pathogens. Hence, it is significant to explore the impact of rainfall carrying the live microbial on the quality of freshwater reservoirs in terms of changes in the abundance of pathogens, the microbial community composition and diversity.

Drinking water Microbiome Analysis

Understanding and evaluating major bacteriological changes in drinking water treatment and distribution systems is of primary importance for human health. Advancement in 'omics' technologies   promise to reveal new insights into drinking water microbiomes and improve design of engineering interventions and monitoring tools to lower public health risk.

Technical Platforms

With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis various aquatic environmental microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.

Illumina Hiseq Illumina Miseq Ion PGM PacBio SMRT system
Gut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome Analysis
Nanopore system PCR-DGGE Real-time qPCR Clone library
Gut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome Analysis

What Can Creative Biogene Do?

Creative Biogene offers extensive microbiome analysis portfolio includes not only consultancy, study design, sampling solutions and sequencing, but also downstream bioinformatic and biostatistical analyses. And we offer full-service for microbiome which has been successfully achieved involve in various aquatic samples, such as sewage(wastewater), freshwater lake, rainwater, drinking water, and so on.

Project Workflow

High-throughput sequencing analysis process

Fig.1 High-throughput sequencing analysis process

High-throughput sequencing analysis process

Fig.2 PCR-DGGE analysis process

Service Specifications

Sample requirements and preparation

• Sample: Genomic DNA
• Quality requirements: ≥ 300 ng, ≥ 10 ng/μL, OD260/280=1.8-2.0, non-degradative
• Shipment: Dry ice or ice packs
• Repeated freeze-thaw cycles should be avoided

Detectable Objects

River, lake, rainwater glacier meltwater, drinking water seawater, sewage

Detectable Microorganisms

• Bacteria
• Fungi
• Viruses

Basic analysis

• Sequence Filtering and Trimming
• Sequence Length Distribution
• OTU Clustering and Species Annotation
• Diversity Index
• Shannon-Wiener Curve
• Rank-Abundance Curve
• Rarefraction Curve
• Multiple Contrast
• Heatmap
• Principal Components Analysis (PCA)

Routine analysis

• Heatmap
• VENN
• Principal Components Analysis (PCA)
• Microbial Community Structure Analysis
• α Diversity Index Analysis
• Matastats Analysis
• Weighted Unifrac test
• CCA/RDA Analysis

Advanced data analysis

• Phylogenetic Tree
• LDA-Effect Size (LEfSe)
• Network Analysis
• Correlation Analysis

Reference
1. Glasl, B., Webster, N., & Bourne, D. (2017). Microbial indicators as a diagnostic tool for assessing water quality and climate stress in coral reef ecosystems. Marine Biology, 164, 1-18.

For research use only.

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