To better understand microbial dynamics, we characterized and compared the bacterial community of water using rRNA surveys, metagenomics, metabolomics, proteomics, which are good tools to elucidate composition of microbial communities residing in various aquatic environments.
Sewage contains diverse microbes, and regular microbiological screening has become key factor to monitoring the wastewater treatment and protecting environmental health.
Freshwater lakes in diverse regions provide indispensable water resources for humans. The microbial communities in freshwater lake ecosystem play a central role in global environmental and public health perspectives.
Excessive rainfall is a critical origin to historical waterborne disease outbreaks owing to mobilization and transport of bacterial pathogens. Hence, it is significant to explore the impact of rainfall carrying the live microbial on the quality of freshwater reservoirs in terms of changes in the abundance of pathogens, the microbial community composition and diversity.
Understanding and evaluating major bacteriological changes in drinking water treatment and distribution systems is of primary importance for human health. Advancement in 'omics' technologies promise to reveal new insights into drinking water microbiomes and improve design of engineering interventions and monitoring tools to lower public health risk.
With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis various aquatic environmental microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.
|Illumina Hiseq||Illumina Miseq||Ion PGM||PacBio SMRT system|
|Nanopore system||PCR-DGGE||Real-time qPCR||Clone library|
Creative Biogene offers extensive microbiome analysis portfolio includes not only consultancy, study design, sampling solutions and sequencing, but also downstream bioinformatic and biostatistical analyses. And we offer full-service for microbiome which has been successfully achieved involve in various aquatic samples, such as sewage(wastewater), freshwater lake, rainwater, drinking water, and so on.
Fig.1 High-throughput sequencing analysis process
Fig.2 PCR-DGGE analysis process
|Sample requirements and preparation |
• Sample: Genomic DNA
|Detectable Objects |
River, lake, rainwater glacier meltwater, drinking water seawater, sewage
|Detectable Microorganisms |
|Basic analysis |
• Sequence Filtering and Trimming
|Routine analysis |
|Advanced data analysis |
• Phylogenetic Tree
1. Glasl, B., Webster, N., & Bourne, D. (2017). Microbial indicators as a diagnostic tool for assessing water quality and climate stress in coral reef ecosystems. Marine Biology, 164, 1-18.