Summary of Enzyme-Producing Microorganism and Application

EnzymeEnzyme-producing microorganismApplication
ɑ-AmylaseBacillus Subtilis
Bacillus Licheniformis
Aspergillus Oryzae
Liquefy Starch/ Desize Fabric
Digestive Additives/ Enzyme-Containing Detergent
Glucamylase/Gluco-AmylaseAspergillus Oryzae
Aspergillus Niger
Rhizopus Oryzae
Produce Glucose/ Fermentation
Brew Beer
IsoamylaseKlebsiella Aerogenes
Bacillus
Starch Processing
Neutral ProteinaseBacillus Subtilis
Aspergillus Oryzae
Leather/Fur/Food Processing/
Make Condiments/Aid Digestion
Diminish Inflammation/Clarify Beer
Alkaline Protease/ AlcalaseBacillus LiceniformisEnzyme-Containing Detergent
PhytaseAspergillus Niger
Pichia Pastoris
Feed Additives
CellulaseTrichoderma Reesei
Aspergillus Niger
Produce Washer Winkle Fabric/Feed Additives/Digest Plant Cell Wall
HemicellulaseTrichoderma
Aspergillus
Rhizopus
Feed Addictives/
Digest Plant Cell Wall/
Produce Xylooligosaccharide
Β- DextranaseBacillus Subtilis
Aspergillus Niger
Penicillium Emersonii
Chaetomium Sp.
Brew Beer/Feed Aaditives
LactaseAspergillus Oryzae
Aspergillus Niger
Aspergillus Oryzae
Diary Industry
(Handle Milk And Whey)
PectinaseAspergillus
Erwinia
Fruit Processing/Clarify Juice and Fruit Wine/Degumming Long Vegetable Fiber
InvertaseSaccharomyces Cerevisiae
Candida Utilis
A. Niger
Making Invert Sugar
Beverage, Confectionary, Bakery, High Fructose Syrup, Artificial Honey, Calf Feed, Food for Honeybees
Chymosin/Rennin/ RennaseRhizomucor Miehei
Mucor Pusillus
Endothia Parasitica
Produce Cheese
LipaseFungi:Aspergillus
Bacteria:Rhizopus
Enzyme-Containing Detergent
Lipid Processing/Bioengineer
Glucose OxidasePenicillium
Aspergillus
Saccharomyces
Food Deoxidization
Glucose Deprivation/Measure
Glucose IsomeraseBacillus Coagulans
Streptomyces Albus
Produce Hfcs (High Fructose Corn Syrup)
Penicillin AcylasePenicillium
Mycete/ Mucedine
Actinomycetes/ Actinomyces
Produce 6-Aminopicillanic Acid

Main fermentation method:

1. SSF(solid-state fermentation): SSF is defined as any fermentation process performed on a non-soluble material that acts both as physical support and source of nutrients in absence of free flowing liquid. The low moisture content means that fermentation can only be carried out by a limited number of microorganisms, mainly yeasts and fungi, although some bacteria have also been used.

MicroorganismsSSF Process
Bacteria 
Bacillus sp.Composting, natto, amylase
Pseudomonas sp.Composting
Serratia sp.Composting
Streptococcus sp.Composting
Lactobacillus sp.Ensiling, food
Clostridium sp.Ensiling, food
Yeast 
Endomicopsis burtoniiTape cassava, rice
Saccharomyces cerevisiaeFood, ethanol
Schwanniomyces castelliEthanol, amylase
Fungi 
Altemaria sp.Composting
Aspergillus sp.Composting, industrial, food
Fusarium sp.Composting, gibberellins
Monilia sp.Composting
Mucor sp.Composting, food, enzyme
Rhizopus sp.Composting, food, enzyme,organic acids
Phanerochaete chrysosporiumComposting, lignin degradation
Trichoderma sp.Composting, biological control,bioinsecticide
Beauveria sp., Metharizium sp. Biological control, bioinsecticide
Amylomyces rouxiiTape cassava, rice
Aspergillus oryzaeKoji, food, citric acid
Rhizopus oligosporusTempeh, soybean, amylase, lipase
Aspergillus nigerFeed, proteins, amylase, citric acid
Pleurotus oestreatus, sajor-caju Mushroom
Lentinus edodesShii-take mushroom
Penicilium notatum, roquefortiiPenicillin, cheese

2. Submerged Fermentation (SmF)/Liquid Fermentation (LF): SmF utilizes free flowing liquid substrates, such as molasses and broths. The bioactive compounds are secreted into the fermentation broth. The substrates are utilized quite rapidly; hence need to be constantly replaced/supplemented with nutrients. This fermentation technique is best suited for microorganisms such as bacteria that require high moisture content. An additional advantage of this technique is that purification of products is easier. SmF is primarily used in the extraction of secondary metabolites that need to be used in liquid form.

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