Strains are often seen as inherently artificial concepts, characterized by a specific intent for genetic isolation. A strain is a genetic variant or subtype of a microorganism (e.g., virus or bacterium or fungus). Microbial strains can be differentiated by their genetic makeup using metagenomic methods to maximize resolution within species. Scientists from Microbiosci, a division of Creative Biogene, have engineered various strains in order to study their behavior.
Optimized strains of E. coli are typically used for metabolic research. E. coli are also often used as a chassis for the expression of simple proteins. These strains, such as BL21, are engineered to minimize protease activity, hence enabling potential for high efficiency industrial scale protein expression, just like our established recombinant pharmaceutical protein strains. E. coli is the most common species for prokaryotic strain engineering. Our scientists have succeeded in establishing viable minimal genomes from which new strains can be developed. These minimal strains provide a near guarantee that experiments on genes outside the minimal framework will not be effected by non-essential pathways. Besides, yeasts are the most common subjects of eukaryotic strain engineering, especially with respect to industrial fermentation. Moreover, in the case of complex proteins including biologics, mammalian strains are typically used for expression.
With specifically developed micro-organisms such as bacteria, yeasts, fungi and/or micro-algae we can realise a variety of biosynthetic processes. For example, specific chemicals can be developed in a biosynthetic instead of a chemical way (or extraction from crude oil). We can also convert components from low-cost substrates and side streams into energy sources such as hydrogen. Cultures from the Microbiosci Bacteriology Collection are useful in a variety of research and industrial and agricultural applications, and include methicillin-resistant/sensitive research materials, quality control organisms for commercial identification systems, a wide selection of extremophile strains from a variety of environmental sources, and genomic DNA from well-characterized microbial strains suitable for amplification by PCR.