Staphylococcus Surface Display Service

In general, many surface proteins of Gram-positive bacteria are covalently attached to the cell wall. Staphylococcal protein A (SpA) is a 40–60 kDa surface protein which has often been used as a model surface display system to study anchoring mechanisms. Staphylococcus aureus is a Gram-positive, round-shaped bacterium that secret surface proteins across septal membranes for assembly into the bacterial cross-wall. Surface proteins of Staphylococcus aureus play a very crucial role in the pathogenesis of human infections and are anchored to the cell wall envelope by sortases.

Staphylococcus Surface Display Service

Fig.1 cell surface display in Gram-positive bacteria

A suitable anchoring protein needs to meet the following requirements:

• They must have a strong anchoring domain to ensure that exogenous proteins can be immobilized on the surface.
• They must be compatible with foreign proteins, be able to form fusion proteins, and should not be able to interact with each other.
• They must be resistant to protease hydrolysis.

Four major types of cell surface displayed proteins are currently recognized:

• Transmembrane proteins
• Lipoproteins
• LPXTG-like proteins
• Cell wall binding proteins

Moreover, Staphylococcus carnosus exhibit several traits attractive for display of functional proteins on the surface compared to Gram-negative bacteria.

• Single cell membrane architecture and translocation through only one membrane
• The Gram-positive bacteria have been shown to be more rigid
• The cell is viable in harsh conditions and thus suitable for high-speed flowcytometric sortings.

Advantages of Our Services

• We have many years of service experience in cell surface display.
• We have a world-class service platform.
• The price of Creative Biogene is very competitive in the industry and the price is favorable.
• One-stop service.
• Ensure efficient service, and urgent service can be provided.
• Periodic progress updates.

Assay Validation:

• SDS-PAGE analysis
• Western Blot analysis
• Enzyme assays
• Fluorescence assays
• Flow cytometry
• Immunofluorescence microscopy

Turnaround Time

In general, our turnaround time is 4-8 weeks depending on the size of your project.

Creative Biogene offers staphylococcus surface display service to help you display the protein you are interested. With the rich experience, our talented scientists advise appropriate construction strategy in various biotechnological applications. Please feel free to contact us if you have any concerns. Know more about how our experienced team can facilitate your work, please feel free to contact us at .

Reference
1. Lee, S. Y., Choi, J. H., & Xu, Z. (2003). Microbial cell-surface display. Trends in Biotechnology, 21(1), 45-52.

For research use only.

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