Skin Microbiome Analysis

Skin, which is the largest organ of the body, forms a critical protective barrier between a mammal and its external environment. The human skin provides home to millions of bacteria, fungi and viruses, which play extremely important role to the skin health and protection against external infectious or toxic substances.

Characterization of skin microbiota is essential for diagnosing skin conditions. Interacting symbionts and immunity in the skin predict disease risk and probiotic effectiveness. Skin microorganisms also produce compounds that influence host physiology. Investigation of the skin microbiome provides opportunity to understand the microbial involvement in human skin disorders and to enable novel promicrobial and antimicrobial therapeutic approaches for their treatment.

Skin Microbiome Analysis

Potential Research Directions of Skin Microbiota Analysis

• Explore the differences in skin microbiota composition and content between the healthy state and in a variety of disease states
• Explore the differences in skin microbiota composition and content during the development of a certain disease to search for disease-related biomarkers
• Explore the differences in skin microbiota composition and content at different drug or diet intervention stages
• Explore the differences in skin microbiota composition and content at different developmental stages of the same individual
• Explore the differences in skin microbiota composition and content at different body parts of the same individual

Technical Platforms

With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis the skin microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.

Illumina Hiseq	Illumina Miseq	Ion PGM	PacBio SMRT system

Nanopore system	PCR-DGGE	Real-time qPCR	Clone library

What Can Creative Biogene Do?

Creative Biogene offers complete solutions to demonstrate the real efficacy of your product on the microbiome, which include but not limited to cosmetics, soaps, personal care products, antibiotics, probiotics or other interventions.

Project Workflow

High-throughput sequencing analysis process

Fig.1 High-throughput sequencing analysis process

High-throughput sequencing analysis process

Fig.2 PCR-DGGE analysis process

Service Specifications

Sample requirements and preparation • Sample: Genomic DNA • Quality requirements: ≥ 300 ng, ≥ 10 ng/μL, OD260/280=1.8-2.0, non-degradative • Shipment: Dry ice or ice packs • Repeated freeze-thaw cycles should be avoided
Detectable Microorganisms • Bacteria • Fungi • Viruses
Basic analysis • Sequence Filtering and Trimming • Sequence Length Distribution • OTU Clustering and Species Annotation • Diversity Index • Shannon-Wiener Curve • Rank-Abundance Curve • Rarefraction Curve • Multiple Contrast • Heatmap • Principal Components Analysis (PCA)	Routine analysis • Heatmap • VENN • Principal Components Analysis (PCA) • Microbial Community Structure Analysis • α Diversity Index Analysis • Matastats Analysis • Weighted Unifrac test • CCA/RDA Analysis	Advanced data analysis • Phylogenetic Tree • LDA-Effect Size (LEfSe) • Network Analysis • Correlation Analysis

References
1. Byrd, A., Belkaid, Y. & Segre, J. The human skin microbiome. Nat Rev Microbiol 16, 143–155 (2018).
2. Nina N. Schommer, Richard L. Gallo, Structure and function of the human skin microbiome,Trends in Microbiology, Volume 21, Issue 12, 2013,

For research use only.

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