Recombinant Vaccine Production

A vaccine typically contains an agent (or several agents) that resembles an associated structure of disease-causing microorganism, which is often made from weakened or killed forms of the microbe, its toxins, or its surface proteins. Advances in immunology, biochemistry, molecular biology, proteomics and genomics have added new perspectives to the vaccinology field. The use of highly purified recombinant proteins allows the targeting of immune responses focused against few protective antigens without the potential risks which might come along with traditional vaccines such as attenuated and natural macromolecules. A recombinant vaccine is produced through recombinant DNA technology, which involves inserting the DNA encoding an antigen that stimulates immune response into microbial or animal cells. The purified protein can be used for vaccination to stimulate the body's immune system, the body would recognize the agent as a threat and destroy it, then further recognize and eliminate any of the microorganisms associated with that agent that it may encounter in the future.

Recombinant Vaccine Production

Figure 1. Manufacturing Recombinant Vaccines

In the manufacturing process of a vaccine, one or several genes of etiologic agents have been cloned, expressed and purified to be developed as vaccines. There are a variety of expression systems with different advantages such as bacteria, yeast, mammalian cells and insect cells, allowing the production of large quantities of proteins depending on the required characteristics, which would stimulate the immune system in a way similarly to bearing natural infections. And the vaccines can be put into use for both prophylactic and therapeutic.

Application & Advantages

Creative Biogene is a premier institution offering professional and comprehensive recombinant vaccines production service. We have years of experience to add value to your program of recombinant vaccines production. Our service covers various vaccines for but not limit to:

• Hib (Haemophilus influenzae type b) disease
• Hepatitis B
• HPV (Human papillomavirus)
• Whooping cough (part of the DTaP combined vaccine)
• Pneumococcal disease
• Meningococcal disease
• Shingles 

We offer the full service of manufacturing with advanced genetic tools and fermentation techniques – Yeast and Escherichia coli, as the most studied expressing systems, have been the favorite microorganisms for the production of vaccines by means of recombinant DNA technology. They are the most thoroughly studied and best-understood organisms in the living world because of the wealth of information on their genetics, biochemistry, and physiology. Furthermore, they are easy to culture in large amounts in economical media and multiply very rapidly. Recombinants genes that code for the vaccines are transferred into the recipient cells by suitable vectors. Though bacterial expression systems are the most used expressing systems because of the ease of handling and high-level expression, when it comes to recombinant vaccines that post-translational modifications are necessary, mammalian or insect cells should be considered.

Creative Biogene can also provide you with the services to the evaluate the expression level of each specific expression vector and promoter, as well as the presence or absence of post-translational modification by the recombinant vector which are both essential features that influence the safety of recombinant vaccines and efficacy of their production. The optimization of fermentation condition will also be performed to ensure quality and productivity of the production. Creative Biogene dedicates in improving the manufacturing processes to offer an optimized and cost-effective scheme. Simply let us know your requirement. We will make every effort to meet your needs!

See also:

Techniques used in recombinant pharmaceutical protein

E.coli Expression System

Yeast Expression System

E.coli / Yeast Genome Editing 

Strains list for recombinant pharmaceutical protein production

E.coli Strain

Yeast Strain

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