Coagulation is the process that blood congealing from a liquid to a gel, forming a blood clot. It potentially results in hemostasis, ceasing blood loss from a damaged vessel, followed by repairing. The mechanism of coagulation involves activation, adhesion and aggregation of platelets, as well as deposition and maturation of fibrin. Coagulation factors, also known as human blood clotting factors, are mostly proteins (except for coagulation factor IV) circulating in the blood that are essential for proper blood clot formation. Coagulation is a complex process that involves numerous coagulation factors, which are mostly produced by the liver and blood vessels in a human body. Numbered according to the order of their discovery, 13 categories of coagulation factor have been recognized by scientists so far:
|III||Tissue factor or thromboplastin|
|VIII||Antihaemophilic factor A, Antihaemophilic globulin|
|IX||Antihaemophilic factor B, Plasma thromboplastin component, Christmas factor|
|XI||Plasma thromboplastin antecedent, Haemophilia C, Rosenthal syndrome|
|XIII||Fibrin stabilising factor, Laki-Lorand factor|
Disorder of coagulation can result in hemorrhage, bruising, or thrombosis, one of the well-studied diseases is hemophilia - a coagulation factor deficiency, in most cases, caused by inherited genetic disorder. The two main types of haemophilia – haemophilia A and haemophilia B, occur due to low amounts of coagulation factor VIII and coagulation factor IX respectively. Coagulation factor transfusions are vital treatment approaches for people with such coagulation factor deficiency or malfunction diseases. Though in the early days transfusions with pooled plasma might lead to serious blood-borne infection, together with the problem of limited acquisition.
In the late 20th century, the development of recombinant DNA technology brought the production of pharmaceutical coagulation factors to a new era. The biological tools that we utilize for the production of coagulation factors include microorganisms, plant cells and mammalian cell lines (such as CHO, BHK and HEK-293), among which microbial expression systems are widely used in coagulation factor production due to the intrinsic advantages including rapid growth, high yields and easy manipulation, making them the premier choice for expression of recombinant coagulation factors.
Creative Biogene is the premier institution offering professional and comprehensive recombinant coagulation factors production services. We have years of experience to add value to your research and manufacturing. We offer the full service of manufacturing recombinant coagulation factors (such as factor VII, factor VIII, factor IX, factor XII, factor XIII, etc.) with advanced genetic tools and fermentation techniques – Yeast (especially Pichia pastoris and Saccharomyces cerevisiae) and Escherichia coli, as the most studied expressing systems, have been the favorite microorganisms for the production of coagulation factors by means of recombinant DNA technology. They are the most thoroughly studied and best-understood organisms in the living world because of the wealth of information on their genetics, biochemistry, and physiology. Furthermore, they are easy to culture in large amounts in economical media and multiply very rapidly. Recombinants genes that code for the coagulation factors are transferred into the recipient cells by suitable vectors. After the construction of strains, the optimization of fermentation condition will be performed to ensure quality and productivity of the production.
We dedicate in improving the manufacturing processes to offer an optimized and cost-effective treatment for the patients. Simply let us know your requirement. We will make every effort to meet your needs!
Techniques used in recombinant pharmaceutical protein
Strains list for recombinant pharmaceutical protein production