Pseudomonas Fluorescens Gene Expression System

Creative Biogene is a leading company offering high-quality Pseudomonas Fluorescens gene expression service for our esteemed clients around the world across both academic institutions and biotech/pharmaceutical industries. With our strong technical force, unparalleled customized service and fast delivery time, Creative Biogene specializes in providing more cost-effective gene expression service to accelerate the global research in microbial enzymes.

A bottleneck to product development can be reliable expression of active microbial enzymes1. Pseudomonas fluorescens is a biosafety level 1 organism that has been used for high yield production of proteins for agricultural applications and therapeutics specifically. Secondly, the system's performance is due to the combination of a robust host strain, the availability of extensive molecular biology and bioinformatics tools, and a well optimized high cell density fermentation process. Moreover, it allows more ready identification of novel regulatory signals that can direct secretion of high levels of protein to the periplasm offers flexibility to identify the best secretion leader.

Fig.1 The P. fluorescens Strain Engineering Toolbox. (Retallack, et al. 2012)

As a leader in gene expression service, Creative Biogene provides a "one-stop shop" tailored to your microbial enzymes research needs. Our services range from sequence optimization and synthesis, plasmid construction, induce, flexible scale microbial enzymes purification and characterization, post processing of purified protein et al. It's entirely customizable and flexible to fit how you run projects. Our vision is to provide superior and affordable Pseudomonas Fluorescens gene expression service to the academic and industrial scientific research communities.

Workflow of Pseudomonas Fluorescens gene expression service

As state-of-the-art microbial enzymes solutions provider, Creative Biogene has a proprietary technology platform and an international network to provide our clients with professional and comprehensive solutions and applications. Creative Biogene specialize in Pseudomonas Fluorescens gene expression system. Simply let us know the gene to be expressed. We will propose the best strategy tailored to your requirements.

References
1. Retallack, D. M., Hongfan, J., & Lawrence, C. (2012). Reliable protein production in a pseudomonas fluorescens expression system. Protein Expression & Purification, 81(2), 157-165.
2. Retallack, D., Schneider, J. C., Chew, L., Ramseier, T., Allen, J., & Patkar, A., et al. (2006). Pseudomonas fluorescens – a robust expression platform for pharmaceutical protein production. Microbial Cell Factories, 5(S1), S28.

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