The novel coronavirus SARS-CoV-2/2019-nCoV is an enveloped, non-segmented, positive sense RNA virus that has four main structural proteins [Spike (S)-Envelope (E)-Membrane (M)-Nucleocapsid (N)]. The spike (S) protein, a main target for neutralization antibody, is crucial for determining host tropism and transmission capacity by attraction with angiotensin-converting enzyme 2 (ACE2). Generally, spike glycoprotein is functionally cleaved by the host cell furin-like protease into S1 domain and S2 domain. The S1 domain is responsible for receptor binding while S2 domain functions to mediate virus fusion in transmitting host cells.
Fig.1 Genome-wide structure and function modeling of SARS-COV-2
(The Yang Zhang Lab - University of Michigan)
Vaccine is a biological preparation that is recognized worldwide as one of the most effective ways for combating infectious diseases. Recombinant protein-based vaccines ang probiotics-based oral vaccines are new approaches to prevent COVID-19 caused by SARS CoV2 and other coronaviruses of pandemic potential. Furthermore, novel strategies based on food grade vaccine formulations have been demonstrated to trigger both a systemic and mucosal immune response.
Fig.2 Food grade vaccines
As a biotechnology company, Creative Biogene have expertise in genome editing (knock in) and gene expression. Creative Biogene is able to provide you the SARS-CoV-2 protein yeast expression service, including yeast, lactic acid bacteria, but also alternative systems such as other bacteria than E. coli, B. subtilis, etc. If you require any further information, please feel free to contact us at email@example.com.
1. Astuti, I., & Ysrafil (2020). Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): An overview of viral structure and host response. Diabetes & metabolic syndrome, 14(4), 407-412.
2. Criscuolo, E. , Caputo, V. , Diotti, R. A. , Sautto, G. A. , Kirchenbaum, G. A. , & Clementi, N. . (2019). Alternative methods of vaccine delivery: an overview of edible and intradermal vaccines. Journal of Immunology Research, 2019, 1-13.