Microbial Oil Production

The yearly dropping fossil fuel storage and the consequent environmental problems have brought energy issue to the forefront of the world. Bioenergy derived from biomass plays a more and more significant role in today’s energy supply system due to its renewability and sustainability. Microbial oil, a triglyceride produced by oleaginous microorganisms, is similar to other kinds of biodiesels such as vegetable oil and animal fat-based diesel consisting of long-chain alkyl, which can be used in standard diesel engines, thought, much more acquirable. The production of biodiesel through microorganism could be a quite promising approach to alleviate the dilemma we are facing as to energy shortage.

Microbial oil, also known as single cell oil, is generally extracted from some yeast, molds, microalgae and bacteria, which utilize carbohydrate, hydrocarbon and/or ordinary grease as carbon sources and produce oil and/or lipids with commercial value. Due to its higher degree of unsaturation than animal and vegetable oil, microbial oil is even more qualified as the source of biodiesel. The microbes most frequently used in the fermentation are as follows:

Yeast: Candida sp., Cryptococcus sp., Lipomyces starkeyi, Rhodotorula giutinis, Lipomyces lipofer, Myxozyma sp., etc.

MoldsMortierella isabelline, Mortierella alpine, Mucor circinelloides, Aspergillus oryzae, etc.

Microalgae: Scenedesmus obliquus, Phaeodactylum tricornutum, Chlorella sp., Dunaliella salina, etc.

Microbial Oil Production

Figure 1. Flow chart of the process to convert lignocellulosic biomass into microbial lipids (Guerfali 2017)

The productivity is well influenced by culture conditions including but not limited to culture media composition, pH value, temperature, light condition and ventilatory capacity. With great effort of our marvelous scientists and engineers, the yield can reach up to 70% of lipids in dry biomass with conversion rate above 95%! We will provide meticulous service for every step of the workflow to boost production:

• Strain selection: We provide service with techniques including mutagenesis and random screening, high-throughput assay for rapid screening, DNA recombination, etc.

• Raw material: Selecting most economic raw material and optimizing pretreatment procedure for the strains or the other way around.

• Fermentation condition: Optimizing fermentation condition from beginning to magnification to guarantee smooth production with minimum energy cost.

• Pretreatment of microbes: Methods such as grinding, acid and heat, multigelation, ultrasonic disruption, enzymolysis.

• Extraction: Methods such as organic solvent extraction, Soxhlet extraction, supercritical carbon dioxide fluid extraction.

• Refining: Methods such as acid catalyzed transesterification, alkali catalyzed transesterification, enzyme catalyzed transesterification, subcritical transesterification and supercritical transesterification.

We dedicate in providing service and products of microbial oil with top-level quality, like meeting the requirements of ASTM D6751 - Standard Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels. If you have any special requirements, please feel free to contact us, Creative Biogene will be your trustworthy helper!

1. Guerfali M.; et al. Single cell oil production by Trichosporon cutaneum and lignocellulosic residues bioconversion for biodiesel synthesis. Process Safety and Environmental Protection, 2017, 113.

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Microbial Fermentation

• Microbial Fermentation Service

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