• 45-1 Ramsey Road, Shirley, NY 11967, USA
  • 1-631-626-9181

Microbial Cell-surface Display Service

Microbial cell surface display technology holds great strategic potential for expressing heterologous proteins or polypeptides with a distinct function on the surface of microbial cells. To gain amenable cell surface exposure, the passenger protein could be fused with various anchoring motifs (carrier protein), which are usually cell-surface proteins or their fragments. Up to the characteristics of passenger and carrier proteins, this strategy can be carried out using different fusion methods, such as C-terminal fusion, N-terminal fusion or sandwich fusion. Microbiosci provides various cell-surface display systems for the successful display of a target protein or peptide on the surface of bacteria via genetic fusion to anchoring motif.

Microbial Cell-surface Display Service

Fig.1 fusion strategy of cell surface display

Surface display systems developed for bacteriophages (phage)

Phage display has been used as a high-throughput screening technology which allows the presentation of peptide and protein libraries on the surface of phage with many remarkable advantages.

Surface display systems developed for Gram-negative bacteria

For Gram-negative bacteria, including E. coli, the fragility of outer membrane caused by the display of proteins can be a problem. Nevertheless, E. coli is still an attractive host because it has the advantage of high transformation efficiencies and the availability genetic tools and mutant strains.

Optional anchoring motifs for the enriched display of a target of interest

• Outer membrane protein:OmpA/OprF/LamB
• Lipoprotein:PAL/TraT/Opr1
• Passenger protein:AIDA-I
• S-layer protein
• Ice nucleation protein (INP)
• Pullulanase

Surface display systems developed for Gram-positive bacteria

For Gram-positive bacteria surface display, Bacillus and Staphylococcus strains have been used most often. Gram-positive bacteria seem to be more suitable for whole-cell catalysts and whole-cell adsorbents owing to the rigid structure of outer cell walls. Meanwhile, proteins are presented on the surfaces only through a single membrane layer, avoiding negative influences during the traverse through the periplasmic space and the integration on the outer membrane.

Optional anchoring motifs for the enriched display of a target of interest

• Cell wall binding protein:SPA/M6
• Cell membrane anchor protein:DppE
• Cell surface related protein: SLH

Surface display systems developed for yeast

For yeast surface display, Saccharomyces cerevisiae has been used most often. Yeast surface display has two extraordinary advantages relative to phage and bacteria display technologies. First, yeast is a eukaryotic expression system capable of incorporating post-translational modifications. Second, the heterologous protein can be fused at anchor protein without disrupting its core structure, thus without undermining its surface displaying efficiency.

Microbial Cell-surface Display Service

Fig.2 Applications of microbial cell surface display

The microbial cell surface display technology bears considerable advantages:

• Maintain the relatively independent spatial structure and biological activity of the protein
• The protein is more active and stable
• Greatly simplify the preparation process of the protein
• Reduce the production cost

Advantages of Our Services

• We have many years of service experience in cell surface display.
• We have a world-class service platform.
• The price of Microbiosci is very competitive in the industry and the price is favorable.
• One-stop service.
• Ensure efficient service, and urgent service can be provided.
• Periodic progress updates.

Assay Validation:

• SDS-PAGE Analysis
• Western Blot Analysis
• Enzyme assays
• Fluorescence assays
• Flow cytometry
• Immunofluorescence microscopy

Turnaround Time

In general, our turnaround time is 4-8 weeks depending on the size of your project.

Microbiosci provides various cell-surface display systems for the successful display of a target protein or peptide on the surface of bacteria via genetic fusion to anchoring motif. Microbial genome editing technology could also be employed to generate sandwich fusion for further constitutive expression and surface display. Microbiosci could verify the epitope by SDS-PAGE, western blotting, flow cytometry, and immunoelectron microscopy of the intact bacteria. Know more about how our experienced team can facilitate your work, please feel free to contact us at or 1-631-626-9181.

References
1. Lee, S. Y., Choi, J. H., & Xu, Z. (2003). Microbial cell-surface display. Trends in Biotechnology, 21(1), 45-52.
2. Chen, T. , Wang, K. , Chi, X. , Zhou, L. , Li, J. , & Liu, L. , et al. (2019). Construction of a bacterial surface display system based on outer membrane protein f. Microbial Cell Factories, 18(1).
3. Andreu, C., & del Olmo, M. lí. (2018). Yeast arming systems: pros and cons of different protein anchors and other elements required for display. Applied Microbiology and Biotechnology, 102(6), 2543-2561.

For research use only.

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