As a global leader in recombination-mediated genome editing service, Microbiosci is dedicated to provide a number of services to achieve scientific results faster than ever before. We have a team of passionate and talented scientists who have years of experience and would work closely with you to provide help in reducing your workload while raising the visibility of your research. In addition, relying on Microbiosci's proprietary technologies and mature platform, we offer microbial genome site-directed mutagenesis, gene knock out and Knock-in/replacement service for a broad range of microbes.
The recombineering is a highly efficient and versatile manner of scarless genetic engineering which has the various applications in pushing the boundaries of synthetic biology. The recombineering can modificate nucleotide-precise DNA at any desired position without limitation to restriction sites, since the sequence of the homology regions can be selected casually. Furthermore, the recombineering is appropriate for a diversity of application, for instance introducing of point mutations into open reading frames and insertion or deletion of DNA fragments.
Figure 1. The Schematic of Homologous Recombination
Microbiosci uses the recombinant suicide plasmids manner and Lambda Red (λ-Red) recombinase system to obtain genetic modification successfully. With the accumulation of technology and resource, microbe genomic reconstruction is achieved by interrupting, inserting or deleting genes on the microbial genome to achieve research and utilization of genes.
Workflow of suicide plasmids genome editing services
Workflow of λ-Red recombinase system genome editing services
Advantages of Recombineering Technology
• Easy subcloning and modification of microorganisms
• Fast & Simple & Efficient & Flexible
• Independent of restriction sites
• No size limit
• Precise mutation
Recombineering has enabled faster, more efficient engineering than previous techniques and become a principal choice for genome editing in microbes. Microbiosci 's microbe targets are including but not limited to E. coli, Salmonella, Proteus mirabilis, Enterobacter sakazakii, Enterobacter cloacae, Staphylococcus, Chains Cocci, Lactobacillus, Pseudomonas, and so on. Simply let us know how you want the genome to be edited. We will propose the best strategy for you.