As we all known, Food may be a driver of various human microbiome variation. Microorganisms and their communities on foods are important determinants and indicators of food safety and quality, affecting our ability to detect and identify pathogens during foodborne outbreaks. Microbiome sequencing is useful to characterize complex food microbial communities and uncover characteristics about the associated microbial content. 16S amplicon sequencing (microbial diversity), Metagenomics (microbial community) and metatranscriptomics approaches will surely advance our understanding of how to effectively use the invaluable microbial resources to improve food quality and safety.
• Investigate pathogenic and spoilage microorganisms to study microbial origin and population dynamics of the complex process
• Detect multiple foodborne pathogens and monitor their relative abundances
• Profile particular microbiome constituents such as antimicrobial resistance genes (ARGs), or resistomes
• Monitor the microbiome for food safety and quality using deep shotgun sequencing
With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis the food microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.
Fig.1 High-throughput sequencing analysis process
Fig.2 PCR-DGGE analysis process
|Sample requirements and preparation |
• Sample: Genomic DNA
|Detectable Microorganisms |
|Basic analysis |
• Sequence Filtering and Trimming
|Routine analysis |
|Advanced data analysis |
• Phylogenetic Tree
1. Li, S., Mann, D. A., Zhang, S., Qi, Y., Meinersmann, R. J., & Deng, X. (2020). Microbiome-Informed Food Safety and Quality: Longitudinal Consistency and Cross-Sectional Distinctiveness of Retail Chicken Breast Microbiomes. mSystems, 5(5).
2. Johnson, A. J., Zheng, J. J., Kang, J. W., Saboe, A., Knights, D., & Zivkovic, A. M. (2020). A Guide to Diet-Microbiome Study Design.Frontiers in nutrition, 7, 79.
3. De Filippis, F., Parente, E., & Ercolini, D. (2018). Recent Past, Present, and Future of the Food Microbiome. Annual Review of Food Science and Technology, 9(1), 589–608.