Creative Biogene is a leading company offering the best CRISPR /Cas9-mediated genome editing service. Our talented scientists have extensive experience in gene knockout, knock-in/replacement and site-directed mutagenesis. They would research closely with you to offer help in microbial genome editing.
Derived from the immune system present in bacteria and archaea, CRISPR technology as an efficient genome-scale editing tool has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering1. So far, the CRISPR/Cas9 system has rapidly become a transformative and powerful tool for editing genomes in many eukaryote organisms, however its application in bacteria remains ineffective and limited. As many bacteria do not possess major repair pathways as eukaryotes do, CRISPR-induced double-stranded breaks (DSBs) are lethal1. To improve recombination rates, combination with the λ-Red recombineering and the CRISPR /Cas9 nuclease systems to realize a variety of highly accurate and scarless genome modifications is a powerful and efficient tool for selecting microbial producer strains and obtaining new metabolic products.
Figure 1. Non-homologous end joining (NHEJ) and Homologous recombination (HR) in DSB repair (Tian, et al. 2017)
As a leader in microbial genome editing, Creative Biogene provides a "one-stop shop" tailored to your gene research needs. We have expertise in gene knockout, knock-in/replacement and site-directed mutagenesis. Our services range from sgRNA vector construction to genome editing, clonal selection and validation. It's entirely customizable and flexible to fit how you run projects.
Workflow of CRISPR/Cas9-mediated genome editing services
Advantages of CRISPR/Cas9 Technology
• High efficiency & precision
• Multigene editing: can knock-out up to 5 genes simultaneously
• Easy selection: no selectable marker is required
Creative Biogene is the premier institution to provide professional and comprehensive genome editing services. We have years of experience to meet your specific project needs in using the CRISPR technology to add value to your research project. Creative Biogene is dedicated to providing complete solutions for genome editing to help you every step of the way in your genome editing workflow. And we offer full-service for genetic modification which has been successfully achieved in microorganisms, such as E. coli, Salmonella, Streptomyces, Clostridium, Bacillus subtilis, cyanobacteria, Saccharomyces cerevisiae, filamentous fungi, and so on. Simply let us know how you want the genome to be edited. We will propose the best strategy for you.
1. Tian, P., Wang, J., Shen, X., Rey, J. F., Yuan, Q., & Yan, Y. (2017). Fundamental crispr-cas9 tools and current applications in microbial systems. Synthetic & Systems Biotechnology, 2(3), 219-225.