The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface such as a tooth, rock, or surface, and may include a single species or a diverse group of microorganisms. The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. Recent rRNA surveys, metagenomics, metabolomics and proteomics technologies promise to reveal new insights into the structure, function and dynamics of the biofilm microbial community.
• Explore the infections associated with the biofilm in medicine
• Explore uses and impact associated with the biofilm in aquaculture
• Explore uses and impact associated with the biofilm in industry
• Explore health risk in biofilm formation on food poses
With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis the biofilm microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.
|Illumina Hiseq||Illumina Miseq||Ion PGM||PacBio SMRT system|
|Nanopore system||PCR-DGGE||Real-time qPCR||Clone library|
Creative Biogene offers extensive microbiome analysis portfolio includes not only consultancy, study design, sampling solutions and sequencing, but also downstream bioinformatic and biostatistical analyses.
Fig.1 High-throughput sequencing analysis process
Fig.2 PCR-DGGE analysis process
|Sample requirements and preparation |
• Sample: Genomic DNA
|Detectable Microorganisms |
|Basic analysis |
• Sequence Filtering and Trimming
|Routine analysis |
|Advanced data analysis |
• Phylogenetic Tree
1. Monroe, D. (2007). Looking for Chinks in the Armor of Bacterial Biofilms. PLoS Biology, 5(11), e307.