Biofilm Microbiome Analysis

The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface such as a tooth, rock, or surface, and may include a single species or a diverse group of microorganisms. The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. Recent rRNA surveys, metagenomics, metabolomics and proteomics technologies promise to reveal new insights into the structure, function and dynamics of the biofilm microbial community.

Biofilm Microbiota Analysis

Potential Research Directions of Biofilm Microbiota Analysis

• Explore the infections associated with the biofilm in medicine 
• Explore uses and impact associated with the biofilm in aquaculture
• Explore uses and impact associated with the biofilm in industry
• Explore health risk in biofilm formation on food poses

Technical Platforms

With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis the biofilm microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.

Illumina Hiseq Illumina Miseq Ion PGM PacBio SMRT system
Gut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome Analysis
Nanopore system PCR-DGGE Real-time qPCR Clone library
Gut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome Analysis

What Can Creative Biogene Do?

Creative Biogene offers extensive microbiome analysis portfolio includes not only consultancy, study design, sampling solutions and sequencing, but also downstream bioinformatic and biostatistical analyses. 

Project Workflow

High-throughput sequencing analysis process

Fig.1 High-throughput sequencing analysis process

High-throughput sequencing analysis process

Fig.2 PCR-DGGE analysis process

Service Specifications

Sample requirements and preparation

• Sample: Genomic DNA
• Quality requirements: ≥ 300 ng, ≥ 10 ng/μL, OD260/280=1.8-2.0, non-degradative
• Shipment: Dry ice or ice packs
• Repeated freeze-thaw cycles should be avoided

Detectable Microorganisms

• Bacteria
• Fungi
• Viruses

Basic analysis

• Sequence Filtering and Trimming
• Sequence Length Distribution
• OTU Clustering and Species Annotation
• Diversity Index
• Shannon-Wiener Curve
• Rank-Abundance Curve
• Rarefraction Curve
• Multiple Contrast
• Heatmap
• Principal Components Analysis (PCA)

Routine analysis

• Heatmap
• VENN
• Principal Components Analysis (PCA)
• Microbial Community Structure Analysis
• α Diversity Index Analysis
• Matastats Analysis
• Weighted Unifrac test
• CCA/RDA Analysis

Advanced data analysis

• Phylogenetic Tree
• LDA-Effect Size (LEfSe)
• Network Analysis
• Correlation Analysis

Reference
1. Monroe, D. (2007). Looking for Chinks in the Armor of Bacterial Biofilms. PLoS Biology, 5(11), e307.

For research use only.

Quick Inquiry

  • Verification code

Creative Biogene is fully engaged in developing unique technologies that provide global scientists with high quality products and satisfactory services to facilitate the investigation of microbial researches.

Subscribe

Enter your email address to subscribe.

Microbial Fermentation

• Microbial Fermentation Service

Copyright © Creative Biogene. All Rights Reserved.