Creative Biogene is a leading company offering high-quality Bacillus subtilis gene expression service for our esteemed clients around the world across both academic institutions and biotech/pharmaceutical industries. With our strong technical force, unparalleled customized service and fast delivery time, Creative Biogene specializes in providing more cost-effective gene expression service to accelerate the global research in microbial enzymes.
As an attractive host，Bacillus subtilis is a nonpathogenic bacterium that can efficiently secrete large quantities of a variety of enzymes into the culture medium. Such secretion facilitates further purification steps and simplifies product recovery. Many Processes concerning production technology and large-scale fermentation using this bacterium is very well acquainted. The major advantage of B. subtilis is that it does not produce LPS. And it has no significant bias in codon usage which is considered as an added advantage. However, some problems could be found down the pipeline, such as instability of plasmids, reduced or non-expression of the protein of interest, and even production of extracellular proteases which recognize and degrade heterologous proteins. Our talented scientists have extensive experience to help you solve all your problems.
Fig.1 Construction of pP43NMK, a B. subtilis expression vector for the production and secretion of MPH (Zhang, et al. 2005)
As a leader in gene expression service, Creative Biogene provides a "one-stop shop" tailored to your microbial enzymes research needs. Our services range from sequence optimization and synthesis, plasmid construction, induce, flexible scale microbial enzymes purification and characterization, post processing of purified protein et al. It's entirely customizable and flexible to fit how you run projects. Our vision is to provide superior and affordable Bacillus subtilis gene expression service to the academic and industrial scientific research communities.
Workflow of Bacillus subtilis Gene Expression Service
• Does not produce LPS/endotoxins
• Can be transformed readily with many bacteri-ophages and plasmids
• Capable of secreting functional extracellular proteins directly into the culture medium
• Regulatory acceptability
• Well-developed fermentation and product recovery technologies
As state-of-the-art microbial enzymes solutions provider, Creative Biogene has a proprietary technology platform and an international network to provide our clients with professional and comprehensive solutions and applications. Creative Biogene specialize in Bacillus subtilis gene expression system. Simply let us know the gene to be expressed. We will propose the best strategy tailored to your requirements.
1. Zhang, X. Z., Cui, Z. L., Hong, Q., & Li, S. P. (2005). High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis wb800. Appl Environ Microbiol, 71(7), 4101-4103.