Air borne Microbiome Analysis

The airborne microbiome has diversity levels similar to other ecosystems. Air is a major source of human microbial exposure and polluted air poses a significant threat to human health. Recent reports provide evidence that airborne microbial communities have the potential effect on human health by respiratory pathogens and agents that trigger allergies. In addition, Air microbial organisms also impact agricultural productivity, as bacterial and fungal species distributed by air movement act as plant blights. Therefore, the airborne microbiome is vital for insights into the atmosphere as an ecosystem, but also will inform on respiratory health aspects. Advancement in 'omics' technologies to characterize airborne microbial communities have greatly expanded our ability to explore the dynamics of microbial organisms.

Air Microbiota Analysis

Potential Research Directions of Airborne Microbiota Analysis

• Explore the composition of aerial microbiomes.
• Explore bioaerosol community composition and function, air microbiomes and human health, pathogen detection and modelling.
• Explore the impact of seasonal and diel changes in the community composition of airborne microorganisms.
• Explore associations between exposure to air pollutants and the compositional and functional profile of the human gut microbiome.

Technical Platforms

With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis the air microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.

Illumina Hiseq Illumina Miseq Ion PGM PacBio SMRT system
Gut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome Analysis
Nanopore system PCR-DGGE Real-time qPCR Clone library
Gut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome AnalysisGut Microbiome Analysis

What Can Creative Biogene Do?

Creative Biogene offers extensive microbiome analysis portfolio includes not only consultancy, study design, sampling solutions and sequencing, but also downstream bioinformatic and biostatistical analyses.

Project Workflow

High-throughput sequencing analysis process

Fig.1 High-throughput sequencing analysis process

High-throughput sequencing analysis process

Fig.2 PCR-DGGE analysis process

Service Specifications

Sample requirements and preparation

• Sample: Genomic DNA
• Quality requirements: ≥ 300 ng, ≥ 10 ng/μL, OD260/280=1.8-2.0, non-degradative
• Shipment: Dry ice or ice packs
• Repeated freeze-thaw cycles should be avoided

Detectable Microorganisms

• Bacteria
• Fungi
• Viruses

Basic analysis

• Sequence Filtering and Trimming
• Sequence Length Distribution
• OTU Clustering and Species Annotation
• Diversity Index
• Shannon-Wiener Curve
• Rank-Abundance Curve
• Rarefraction Curve
• Multiple Contrast
• Heatmap
• Principal Components Analysis (PCA)

Routine analysis

• Heatmap
• Principal Components Analysis (PCA)
• Microbial Community Structure Analysis
• α Diversity Index Analysis
• Matastats Analysis
• Weighted Unifrac test
• CCA/RDA Analysis

Advanced data analysis

• Phylogenetic Tree
• LDA-Effect Size (LEfSe)
• Network Analysis
• Correlation Analysis

1. Moelling, K., & Broecker, F. (2020). Air Microbiome and Pollution: Composition and Potential Effects on Human Health, Including SARS Coronavirus Infection. Journal of environmental and public health, 2020, 1646943.
2. Ruiz-Gil, T., Acuña, J. J., Fujiyoshi, S., Tanaka, D., Noda, J., Maruyama, F., & Jorquera, M. A. (2020). Airborne bacterial communities of outdoor environments and their associated influencing factors. Environment International, 145, 106156.

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