The airborne microbiome has diversity levels similar to other ecosystems. Air is a major source of human microbial exposure and polluted air poses a significant threat to human health. Recent reports provide evidence that airborne microbial communities have the potential effect on human health by respiratory pathogens and agents that trigger allergies. In addition, Air microbial organisms also impact agricultural productivity, as bacterial and fungal species distributed by air movement act as plant blights. Therefore, the airborne microbiome is vital for insights into the atmosphere as an ecosystem, but also will inform on respiratory health aspects. Advancement in 'omics' technologies to characterize airborne microbial communities have greatly expanded our ability to explore the dynamics of microbial organisms.
• Explore the composition of aerial microbiomes.
• Explore bioaerosol community composition and function, air microbiomes and human health, pathogen detection and modelling.
• Explore the impact of seasonal and diel changes in the community composition of airborne microorganisms.
• Explore associations between exposure to air pollutants and the compositional and functional profile of the human gut microbiome.
With our next-generation sequencing (NGS) technology, PacBio SMRT sequencing, and Nanopore sequencing platforms, we have the tool to identify, quantify and analysis the air microbiota. With this high-throughput technology we can perform complete metagenomics studies capable of surveying the genomes of entire communities, including those of unculturable organisms.
|Illumina Hiseq||Illumina Miseq||Ion PGM||PacBio SMRT system|
|Nanopore system||PCR-DGGE||Real-time qPCR||Clone library|
Creative Biogene offers extensive microbiome analysis portfolio includes not only consultancy, study design, sampling solutions and sequencing, but also downstream bioinformatic and biostatistical analyses.
Fig.1 High-throughput sequencing analysis process
Fig.2 PCR-DGGE analysis process
|Sample requirements and preparation |
• Sample: Genomic DNA
|Detectable Microorganisms |
|Basic analysis |
• Sequence Filtering and Trimming
|Routine analysis |
|Advanced data analysis |
• Phylogenetic Tree
1. Moelling, K., & Broecker, F. (2020). Air Microbiome and Pollution: Composition and Potential Effects on Human Health, Including SARS Coronavirus Infection. Journal of environmental and public health, 2020, 1646943.
2. Ruiz-Gil, T., Acuña, J. J., Fujiyoshi, S., Tanaka, D., Noda, J., Maruyama, F., & Jorquera, M. A. (2020). Airborne bacterial communities of outdoor environments and their associated influencing factors. Environment International, 145, 106156.